Can you please comment on how happy /not happy you are with it?
Yes, same with ours. It was practically new and it just broke. Thanks for the feedback !
Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...
19 July 2024 3,094 2 View
which publication do you recommend for submitting an article on the philosophical and sociological analysis of AI vs Society
30 January 2024 3,918 2 View
preprints on various scientific topics
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Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production. I am looking for because we have some good solutions...
24 November 2022 7,258 8 View
I have seen in the literature that short chain poly-lactic acid (polylactide; PLA) oligomers are water soluble. But I am unable to find a commercial source of this type of oligomers. Does anyone...
31 October 2022 1,419 6 View
Can anyone suggest a mercury-free LED light source for fluorescence microscopy? I'm looking for the simplest and most economical replacement for mercury bulbs. Not the complicated stuff with many...
05 October 2022 6,057 3 View
Please only answer if you have done this and do not send me paper links. Thanks. Hi technical Gurus :) So, I am working on a cytotoxicity assay based on the Roche's WST-1 reagent. Every setup is...
15 September 2022 1,611 0 View
For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library? I have learned that the...
04 July 2022 3,655 1 View
Based on the literature analysis it could be concluded that one of the most common composition of synthetic lipids to prepare liposome mixture or model membrane (particularly in the work with...
28 June 2022 9,817 3 View
Hello to all I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this...
21 April 2022 6,157 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View