11 Questions 47 Answers 0 Followers
Questions related from Leonid Ouchanov
Please only answer if you have done this and do not send me paper links. Thanks. Hi technical Gurus :) So, I am working on a cytotoxicity assay based on the Roche's WST-1 reagent. Every setup is...
16 September 2022 1,572 0 View
Hello, I need to amplify a region of about 30 KBs from human genomic DNA. One option is to use long range PCR. If you have done it, could you please recommend a kit? Any other techniques that I...
15 December 2021 2,773 3 View
Hi, My MRS medium requires tween 80 that I don't have. I did try tween 20 and the cultures grew. However, my question is whether this will have any "unintended consequences". From what I can tell...
12 January 2020 8,186 2 View
If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...
19 December 2018 6,381 2 View
Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?
14 December 2018 5,252 5 View
F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...
10 April 2018 6,488 3 View
Or, should I ask, how to identify those intergenic spacer sequences that will be variable enough to allow for species-specific primer design? I know that one needs to blast a few (better many)...
23 March 2018 7,780 2 View
Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....
27 March 2017 3,568 12 View
Can you please comment on how happy /not happy you are with it?
17 May 2016 5,367 1 View
Hello Members of RG Community, I am looking for a collaborator as part of my PhD project in Agricultural Sciences. My goal is to bring in a project that will require proficiency in molecular...
27 April 2016 7,936 3 View
I need to purify protein from the supernatant of bacteria grown in broth. Does anyone know what is the best way to isolate the total protein from the sup?
22 April 2016 1,237 6 View
