I am growing epithelial cells on transwells at ALI. I want to measure the ROS production in response to exposure to cigarette smoke. I was wondering if the following would be an ideal procedure:
Any suggestions/recommendations to the protocol?
Also, does the DPBS used in washing need to contain Mg+ and Ca+ compulsorily?
Even if people have not worked with cells on Transwells, I would be happy to hear about people's experience of working with DCFDA.
Hello
See the links here
Hope help you
Good Luck
http://mcms.unife.it/it/ricerca-1/ambiti-di-ricerca/patologia-oncologia-e-biologia-sperimentale/signaltransductionlab/publications/143.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319729/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031511/
Thanks a lot Saleh Alkarim, these links were indeed helpful.
Pranav You can use the protocol which you suggested. You can use different time points after exposure to cigratte smoke.
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