Akash Sabarwal

5 Questions 39 Answers 0 Followers

Questions related from Akash Sabarwal

How can we isolate, characterise and maintain cancer stem cells from lung cancer cells lines?

From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?

30 March 2018 1,312 6 View

Debris in cell culture ?

I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...

06 October 2016 8,416 9 View

There are different ROS which are produced in cultured cells, DCFDA and DHE both stains and detect these ROS, What are the differences?

How DHE and DCFDA detects ROS differently?

05 October 2016 6,121 4 View

What are the best time points to study epigenetic regulation?

For proteins such as HDACs and DNMSts

14 July 2016 6,634 2 View

How can I induce inflammation in gastric cancer cells using LPS?

Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?

30 June 2015 650 3 View