I am currently planning on performing metabolic flux analysis in plants using isotopic sulfur. but I am stuck on how to replace the normal sulfur-containing nutrients in MS media ( such as magnesium sulfate, copper sulfate etc) with isotopic sulfur(32 S or 34 S) containing nutrients. is there a way to incorporate isotopic compounds in nutrient media? if not Is there any other method that can be used? also, where can I get these isotope-labeled nutrients?
* Sulfur-34S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34) of Proteomic Changes in Pseudomonas fluorescens during Naphthalene Degradation*📷
Florian-Alexander Herbst et all 2013
* Soufi B., Kumar C., Gnad F., Mann M., Mijakovic I., Macek B. (2010) Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis. J. Proteome Res. 9, 3638–3646
* Sulfur and carbon isotopic evidence for metabolic pathway evolution and a four-stepped Earth system progression across the Archean and Paleoproterozoic
Author links open overlay panelJeff R.Havig et all
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