I know I should not reuse it for RNA work, but I've heard reusing for protein work is fine. Do I acid wash and autoclave? If acid wash, which acid and for how long, etc?
Thank you for any suggestions!
Steph
Dear Dr. Kyle,
I think it is advisable to avoid reuse of beads. This is because of the sensitivity of all molecular analytical procedures.
Hi Stephanie,
I can't speak for steel beads but we used to use glass beads to bust up yeast cells in homogenizer bottles to extract protein. We used straight up nitric acid to wash and store the beads. Then wash the beads several times with your homogenization buffer prior to using them. Hope this is helpful.
Good Luck!
Hi Steph,
I know it has been two years since you've asked your question and probably you got the answer you are looking for; however, I wanted to share this with you and anyone asking the same question as yours. Please have a look at this web page "protocol of cleaning the beads" https://biospec.com/beads-guide-lines/cleaning-your-beads
Cheers,
Adel
We (https://www.researchgate.net/lab/Bronwyn-Barkla-Lab) have been re-using Qiagen TissueLyser steel beads for a long for seed protein extraction (https://www.researchgate.net/project/Utilization-of-rapeseed-protein-suitable-for-human-consumption).
Once used, washing with ordinary detergent using tap water makes the beads clean. We used to use MilliQ water in the final wash.
Thanks.
this might be a dumb question but may I ask why beads can't be reused for RNA extraction?
Bruno M Ghersi Maybe it is not suitable, but a good wash and sterilization should be good too (careful to be RNAse free!). We used to acid-wash them and we never had problem with our RNA extractions.
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