Does anyone try renaturating inclusion body with copper sulfate?

Stephanie Rebecca Setijono @Stephanie-Setijono

18 March 2024 0 9K Report

I am currently doing isolation of protein from inclusion bodies. I solubilize the protein with 8M guanidine hydrochloride (Simpson, CSHL, 2010). I this step I saw no pellet anymore in my sample. And then when I put copper sulfate for renaturation process, I started aggregates and I observed too much aggregation. Anyone has comments or suggestion? Thank you.

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Science method

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