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Hi everyone, I plan to perfuse-fix my mice with 4% PFA through the heart, carefully extract the brain and then post-fix the brain overnight in 4% PFA for 12-16 hours. I plan to paraffin embed them...
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We would like to measure Xbp1 splicing in the livers of our mutant mice to determine ER stress. We typically fast mice for 5 hours prior to dissection, but will fasting induce splicing of Xbp1?...
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