How to calculate Leaf Water Conten Index (LWCI)?

I want to calculate Leaf Water Conten Index (LWCI) using Landsat or Sentinel data. I found several usefull formulas that use NIR and SWIR bands. But to estimate LWCI I have to have NIR and SWIR...

16 March 2020 9,322 4 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

18 December 2018 6,267 2 View

Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

13 December 2018 5,140 5 View

Monoclonal antibody purity?

If monoclonal antibody would be obtained from ascites fluid during hybridoma expression in vivo -> protein A/G-sepharose purification, how much non-specific AB contamination and non-target...

04 December 2018 6,582 3 View

Does the plasmonic near field spread on the distance sufficient for the resonance light absortion (scattering)?

Cross section of light absorption and light scattering for noble metal (silver, gold) nanoparticle is higher than its geometrical cross section in case of plasmon resonance. It can be...

30 October 2018 5,235 3 View

Degenerate Primers to ITS Region (16S-23S rRNA) for NGS?

F: 50 -CTKTTGGGYYCCCKGRYYG-30 Rev: 50-CGCGTCCACTMTCCAGTTCTC-30 These are primers for NGS sequencing of bifidobacteria from a publication. If you substitute, for example K, with G or T then...

09 April 2018 6,351 3 View

How to obtain a precipitated powder of Poly-Amic Acid based on aromatic diamine and aromatic dianhydride?

How to obtain a precipitated powder of Poly-Amic Acid (PAA) based on aromatic diamine and aromatic dianhydride? It is well known that for several Aromatic Polyimides (PIs) in the course of...

22 December 2017 9,399 3 View

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film ?

Which solvent or a combination of solvents could you propose for controlled swelling of Cellophane film

17 August 2017 1,331 1 View

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know?

I need dataset (training set) for image stegoanalysis on a base of neural networks. Are there such bases? Does anybody know? I know about stegoDB but can't find download link. Thanks

10 April 2017 8,150 9 View

Is that C. jejuni?

Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....

26 March 2017 3,470 12 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View