We used 100 kDa Amicon Ultra 0,5 mL for separation of 70 nm-sized nanocapsules from unbound drug and unfortunately we still recovered some nanocapsules in the lower compartment. We would appreciate any help in this respect.
we follow Table 1 when choosing the pore of the ultrafiltration tubes. https://www.sigmaaldrich.com/technical-documents/articles/biology/nanoparticle-ultrafiltration.html