Are you familiar with isothiocyanate determination protocol?

Can anyone please share the method for total isothiocyanate determination in food and urine samples? Thanks.

04 May 2015 4,907 2 View

Is insulin effective in human myeloid leukemia cells?

I want to activate Akt in human myeloid leukemia cells and insulin treatment is one of the best choices for its activation (Akt phosphorylation). Has anyone ever tried insulin treatment in human...

09 October 2013 7,985 8 View

Is there any transfection reagent for knocking down genes in suspension culture?

Suspension cells are hard to transfect, thus making it difficult to overexpress or knockdown a gene. The alternative strategies are either to do electroporation, nucleofection or lentiviral...

08 September 2013 5,181 42 View

Can someone recommend a comprehensive list of N-Ras mutant cells of human origin?

List of N-Ras mutant cells....

06 July 2013 6,388 7 View

What is the difference between BSA and Non-Fat milk (NFM) blocking of the membranes?

While detecting the phospho-protein people block their NC or PVDF membranes with BSA (3-5%) rather than NFM. Can any one please explain why BSA blocking is preferred for detection of...

06 July 2013 1,408 15 View

Can some one help in explaining the mechanism of Oncogenes amplification in Cancer cells?

Cancer cells have different abnormalities, like mutation or depletion of tumor suppressor proteins, as well as oncogene activation. In many cases cancer cells have amplified genes which make them...

06 July 2013 4,218 2 View

Mycoplasma contamination?

Does anyone know the best method for detection of Mycoplasma contamination in the cell culture system? Also what could be the best approach (drug etc.) to avoid mycoplasma contamination?

04 May 2013 630 13 View

Granulocytic and macrophages differentiation markers?

Can anybody please suggest me the specifc surface proteins (CDs) for asessing the granulocytic and macrophage differentiation of promyleocytic leukemic HL-60 cells?

03 April 2013 8,003 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View