04 April 2013 1 2K Report

I am going to analyze cell surface markers for granulocytic and macrophages differentiation by FACS. what are the specific surface proteins (CDs) that can be asessed?

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Can anyone please share the method for total isothiocyanate determination in food and urine samples? Thanks.

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Is insulin effective in human myeloid leukemia cells?

I want to activate Akt in human myeloid leukemia cells and insulin treatment is one of the best choices for its activation (Akt phosphorylation). Has anyone ever tried insulin treatment in human...

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Is there any transfection reagent for knocking down genes in suspension culture?

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Can someone recommend a comprehensive list of N-Ras mutant cells of human origin?

List of N-Ras mutant cells....

06 July 2013 6,522 7 View

What is the difference between BSA and Non-Fat milk (NFM) blocking of the membranes?

While detecting the phospho-protein people block their NC or PVDF membranes with BSA (3-5%) rather than NFM. Can any one please explain why BSA blocking is preferred for detection of...

06 July 2013 1,545 15 View

Can some one help in explaining the mechanism of Oncogenes amplification in Cancer cells?

Cancer cells have different abnormalities, like mutation or depletion of tumor suppressor proteins, as well as oncogene activation. In many cases cancer cells have amplified genes which make them...

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Mycoplasma contamination?

Does anyone know the best method for detection of Mycoplasma contamination in the cell culture system? Also what could be the best approach (drug etc.) to avoid mycoplasma contamination?

04 May 2013 764 13 View

Granulocytic and macrophages differentiation markers?

Can anybody please suggest me the specifc surface proteins (CDs) for asessing the granulocytic and macrophage differentiation of promyleocytic leukemic HL-60 cells?

03 April 2013 8,166 5 View

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