Is insulin effective in human myeloid leukemia cells?

I want to activate Akt in human myeloid leukemia cells and insulin treatment is one of the best choices for its activation (Akt phosphorylation). Has anyone ever tried insulin treatment in human...

09 October 2013 7,985 8 View

Is there any transfection reagent for knocking down genes in suspension culture?

Suspension cells are hard to transfect, thus making it difficult to overexpress or knockdown a gene. The alternative strategies are either to do electroporation, nucleofection or lentiviral...

08 September 2013 5,181 42 View

Can someone recommend a comprehensive list of N-Ras mutant cells of human origin?

List of N-Ras mutant cells....

06 July 2013 6,388 7 View

What is the difference between BSA and Non-Fat milk (NFM) blocking of the membranes?

While detecting the phospho-protein people block their NC or PVDF membranes with BSA (3-5%) rather than NFM. Can any one please explain why BSA blocking is preferred for detection of...

06 July 2013 1,408 15 View

Can some one help in explaining the mechanism of Oncogenes amplification in Cancer cells?

Cancer cells have different abnormalities, like mutation or depletion of tumor suppressor proteins, as well as oncogene activation. In many cases cancer cells have amplified genes which make them...

06 July 2013 4,218 2 View

Mycoplasma contamination?

Does anyone know the best method for detection of Mycoplasma contamination in the cell culture system? Also what could be the best approach (drug etc.) to avoid mycoplasma contamination?

04 May 2013 630 13 View

Granulocytes and macrophages specific differentiation markers?

I am going to analyze cell surface markers for granulocytic and macrophages differentiation by FACS. what are the specific surface proteins (CDs) that can be asessed?

03 April 2013 1,399 1 View

Granulocytic and macrophages differentiation markers?

Can anybody please suggest me the specifc surface proteins (CDs) for asessing the granulocytic and macrophage differentiation of promyleocytic leukemic HL-60 cells?

03 April 2013 8,003 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View