Dear Muhammad,
You can find a list of N-Ras mutations in different cell lines in the Catalog of Somatic Mutation in Cancer COSMIC http://cancer.sanger.ac.uk/cell_lines/gene/overview?ln=NRAS Under "Mutation Analysis" (right of the screen) click on "distribution" and you should be able to see a table with different links (e.g. Insertion, deletion, substitution coding strand, both strands)-- those links will take you to tables showing different mutations by cell line, location, transcript, histology, etc.
Good luck!
Thanx Rolando Garcia-Milian.........Unfortunately I could not find the names of the specific cell lines there in the table, rather they have given tissue/sources/organs. However, manual search (on the home page) do show detail of all of the mutations in a cell, but selecting one cell at time and looking at the detail is little boring and time consuming.......
If you use the download function you get all the data and can import them into Excel. e.g. File:CosmicCellLineProject_v65_280513.tsv.gz
Hi Muhammad,
They call it "sample names" in the table columns. Dowloading the excel file as Sabine suggest is a great idea since that allows you to filter /analyze the data.
Regards
@Thanx Sabine Strehl and Rolando Garcia-Milian.....I have successfully downloaded the file.....Now its comprehensive....Thanx a lot again....
Lots of AML cell lines contain NRAS mutations, as for example HL60 and THP-1. NB4 contains a KRAS. I always prefer to use the Cancer Cell Line Encyclopedia from the the Broad Institute. Almost all cell lines sequence data is available here.
Thanx Kim Kampen....The website is informative. My work also focuses on HL-60, NB4, THP-1, U937 and K562 cells and I may use some other cells of hematopoietic origin, having no Ras mutation.
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