GAPDH band on western blot showing at a higher molecular weight?

03 July 2023 1 2K Report

Does GAPDH band on Western blot ever comes around 43kDa?

Please see the attached image.

Thank you

There is a streak at your PAGE gel...GAPDH gives a band roughly between 37-40 kDa and this image also indicates a band below 43 kDa...It must be ok, just analyzing inaccurately due to the smile effect you had. You should decrease the loaded total protein amount or (more beneficial) applied voltage to get straight bands, then you can agree the band would be at the correct position. Also, run the gel in a cold environment not let it be heated during the process, this also disturbs the band alignment...

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