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Questions related from İsmail Emir Akyildiz
Is there any way to isolate solely prenylated small molecules from non-prenylated counterparts without employing immunoaffinity interaction?
12 February 2024 8,136 0 View
Is it possible to establish baseline separation for two polymorphic proteins roughly having 50 and 54 kDa MWs by using any polymeric-based SEC column at analytical scale? Any product or shared...
31 May 2023 1,812 2 View
How can we synthesize the silver attached/immobilized polystyrene sulfonate resin? Is there any well-known protocol and/or recipe? I will be much grateful if any articles specific to this...
12 March 2023 1,247 0 View
Hi, Any suggestion about inefficient digestion by BEH particle Enzymate online digestion column? Low flow (100ul/min, increased temperature up to 40 C, varying protein concentrations were tested...
29 November 2022 6,180 1 View
If I do not use total protein normalization, is there any way to perform immunoblot normalization other than housekeeping proteins?.... For the protein extracts from food matrices, without any...
13 November 2022 9,927 1 View
Hi, Is it efficient to use pI precipitation for peptides (actually small proteins; e.g3-4 kDa)? Could you provide references or share your personal experiences?... Although it is a versatile...
14 October 2022 4,920 1 View
Is it possible to eliminate only the bulky fructose and glucose (F/G) content from the matrix of interest simultaneously and selectively? I should get rid of the F/G composition but not the other...
13 February 2022 7,903 5 View
I am trying to synthesis a non-covalent, bulk molecularly imprinted polymer by using precipitation method. Organochloride pesticide was chosen as template and MAA as functional monomer along with...
19 November 2021 4,309 4 View
Is it possible to obtain the fixated and stained target peptide from tricine gel matrix in destained and SDS-free form after separation? It was fixated by 5%glutaraldehyde prior to staining and...
01 October 2021 5,036 0 View
I wonder if there are any disadvantageous sides of using the automated 2D and automated Western systems (e.g. Auto2D® Automated 2-D Gel Electrophoresis Device from Merck for 2D, and Blotcycler /...
11 July 2021 505 2 View
I saw that It has been used once to perform the chemical digestion of the extracellular matrix components. I was wondering that can it be used as a chaotropic agent (alternative to urea/guanidin...
31 May 2021 2,444 2 View
Hi, I've read an application sheet, there was a statement was given below; ''This protocol describes the generation of a cleared lysate from an E. coli cell pellet and the subsequent purification...
02 May 2021 6,628 3 View
Why do we need to prepare the oxime derivates (hydroxylamine.HCL catalyzed) of carbohydrates and organic acids prior to silyl (TMS/hexamethyldisilazane) application for GC analysis? What is the...
05 September 2020 1,293 3 View
What are the pros and cons of the CaptureSelect ™ FcXP Affinity Matrix in antibody isolation compared to protein A and/or protein G matrix? Are there any differences based on affinity and...
19 August 2020 2,974 2 View
Hi Everyone, You see a 2D electrophoresis image of Royal jelly proteome profiling at the attachment. I wonder that can the use of chromatofocusing helps to get similar results? If applicable,...
25 July 2020 9,237 0 View
Hi, I have extracted and concentrated honey enzymes/proteins. Most abundant ones are alpha glucosidases and alpha amylase. Beside of these, other enzymes and major royal jelly proteins are also...
19 February 2020 6,024 1 View
Hi there, I intend to remove carbonate from my %36 guanidinium carbonate solution meanwhile I am planning to get flow through guanidine base solution from SPE cartridge. For this purpose I...
14 February 2020 9,018 0 View
Hello everyone, Could you please suggest a high sensitive oxalic acid and citric acid determination method at urine samples for LC analysis. I intend to perform Mass spec detection at first but...
01 February 2020 2,458 2 View
