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Questions related from İsmail Emir Akyildiz
If I do not use total protein normalization, is there any way to perform immunoblot normalization other than housekeeping proteins?.... For the protein extracts from food matrices, without any...
13 November 2022 9,821 1 View
Hi, Is it efficient to use pI precipitation for peptides (actually small proteins; e.g3-4 kDa)? Could you provide references or share your personal experiences?... Although it is a versatile...
14 October 2022 4,822 1 View
I am trying to synthesis a non-covalent, bulk molecularly imprinted polymer by using precipitation method. Organochloride pesticide was chosen as template and MAA as functional monomer along with...
19 November 2021 4,222 4 View
I wonder if there are any disadvantageous sides of using the automated 2D and automated Western systems (e.g. Auto2D® Automated 2-D Gel Electrophoresis Device from Merck for 2D, and Blotcycler /...
11 July 2021 293 2 View
Hi, I've read an application sheet, there was a statement was given below; ''This protocol describes the generation of a cleared lysate from an E. coli cell pellet and the subsequent purification...
02 May 2021 6,551 3 View
Why do we need to prepare the oxime derivates (hydroxylamine.HCL catalyzed) of carbohydrates and organic acids prior to silyl (TMS/hexamethyldisilazane) application for GC analysis? What is the...
05 September 2020 1,186 3 View
What are the pros and cons of the CaptureSelect ™ FcXP Affinity Matrix in antibody isolation compared to protein A and/or protein G matrix? Are there any differences based on affinity and...
19 August 2020 2,857 2 View
Hi, I have extracted and concentrated honey enzymes/proteins. Most abundant ones are alpha glucosidases and alpha amylase. Beside of these, other enzymes and major royal jelly proteins are also...
19 February 2020 5,955 1 View