I'm working on enzyme beta glucosidase. I used CMC in my production media and the activity starts coming after 5 hours but after 24 hrs no activity is coming. Has anybody experienced the same problem?
Hello, I haven't experienced loss of activity with beta glucosidase but with other enzymes. I will try to help you find some possible reasons for enzyme activity loss. According to some web sources¹²³, some factors that may affect enzyme beta glucosidase activity are:
- Temperature: The enzyme may have an optimal temperature range for its activity, and higher or lower temperatures may reduce or inactivate it. For example, one study¹ found that a beta glucosidase enzyme had maximum activity at 38°C and pH 8.0, and another study² analyzed activity at various temperatures from 20 to 70 °C.
- pH: The enzyme may have an optimal pH range for its activity, and higher or lower pH values may affect its stability or catalytic efficiency. For example, one study¹ found that a beta glucosidase enzyme had maximum activity at pH 5.0, and another study³ found that the enzyme had a broad pH range of 4.0–9.0 with an optimum at pH 5.5.
- Metal ions: The enzyme may require certain metal ions as cofactors or activators for its activity, and the presence or absence of these ions may influence its function. For example, one study¹ found that a beta glucosidase enzyme was activated by Fe2+ and Mn2+, but inhibited by Pb2+ and K+.
- Substrate concentration: The enzyme may have a saturation point for its substrate, and higher substrate concentrations may not increase its activity or may even inhibit it. For example, one study³ found that a beta glucosidase enzyme had a Km value of 0.32 mM for pNPGlc, which indicates its affinity for the substrate.
You may want to check these factors in your experiment and see if they are affecting your enzyme activity.
(1) Identification and characterization of a novel β-glucosidase via .... https://www.nature.com/articles/s41598-017-14073-w.
(2) Impact of host and environmental factors on β-glucuronidase enzymatic .... https://journals.physiology.org/doi/full/10.1152/ajpgi.00026.2020.
(3) Structural and Catalytic Characterization of TsBGL, a β-Glucosidase .... https://www.frontiersin.org/articles/10.3389/fmicb.2021.723678/full.
There is no such thing as an enzyme being active indefinitely. With a Turkish proverb: The ox is dead, the partnership is broken. That is, when the substrate is depleted or the enzyme is saturated, the activity ceases "de facto".
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