Sections with CMC than OCT for spatial transcriptomics slide using Visium HD?

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Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

To what extent Google Scholars Citations system is reliable?

1. I was shocked to discover yesterday that my research citations on Google Scholar had dropped to 2383 from 2419 a week ago, and they are now up to 2391. 2. My citations three months ago were...

28 June 2024 2,846 2 View

Protura for dummies?

Is it possible to tell Proturan families apart based on overall appearance? All the keys I have seen are based on very fine characteristics only readily visible on a slide. Also, there seem to be...

14 June 2024 6,334 0 View

Can Anyone Recommend Papers on OER Mechanism in Perovskites Using DFT?

Hello everyone, I am currently conducting research on the Oxygen Evolution Reaction (OER) mechanisms in perovskite materials and am particularly interested in studies that utilize Density...

03 June 2024 6,295 0 View

Is it necessary to perform both forward and reverse Sanger sequencing to verify the presence of a gene in a sample?

Hello :) I am looking at the presence of toxin biosythensis genes in lake sediments. Nothing fancy, since I only want to verify the specificity of PCR results for primers related to marker genes...

27 May 2024 392 4 View

What is the rationale for both C- Myc tag and 6xHis tag in same plasmid. Can i use only his tag for mammalian expression?

I wanted to express protein in mammalian cells for purification I need 6XHis tag, however, plasmid synthesis company have vector with Myc and his tag. So do I need both or only his tag.

12 May 2024 941 4 View

Methodology for quantifying particulate matter on leaf surface & Pollen by Scanning Electron Microscopy with energy dispersive X-ray spectroscopy?

I needed a detailed methodology for SEM-EDS analysis for Leaf and pollen!

05 May 2024 4,892 5 View

Is it common practice to verify PCR results with Sanger prior to NGS?

Hello, Since a large part of my budget will go on NGS and I don't have the money to repeat it, I want to make sure that it will work. The PCR products look good on the gel (nice bands of the...

21 April 2024 7,785 3 View

What are the currently available options for mass marking ants during an experiment?

Hi all, I'm looking to mass mark Solenopsis xyloni ants during an experiment. For this experiment, I'm allowing the whole colony to feed on three feeders, and I want to mass mark all the ants...

13 April 2024 9,988 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

How are aPTT and PT values reduced after hemodialysis compared to before dialysis value in patients with chronic kidney disease?

I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...

30 July 2024 3,467 0 View

Cell culture model of Acute kidney injury?

Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...

29 July 2024 5,622 3 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

Systematic review meta-analysis paper?

Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...

10 July 2024 5,490 5 View