How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Does increasing antibiotic concentration also increase plasmid production in DH5a E.coli?

Dear all, I am propagating some plasmids with a human gene and an ampicillin resistance gene. My logic is that if ampicillin concentration will be increased, the concentration of plasmid (pSF)...

14 January 2021 779 3 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

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07 December 2020 6,382 2 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View

Which should be used for Anxiety : unselective beta blocker or B1 Blocker ?

which would be more effective in treatment or protection from Anxiety : unselective beta blocker as Propranolol or selective beta-1 adrenergic receptor antagonist as Bisoprolol ?

15 November 2020 7,994 2 View

Effect of excess cyclodextrin on drug after cyclodextrin reaches maximum solubility in phase solubility study?

I am working on phase solubility studies between beta-cyclodextrin and drug? I used more concentration of beta-cyclodextrin till 30mM despite its maximum solubility of 16mM. Usually, above maximum...

30 October 2020 3,921 9 View

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30 October 2020 961 3 View

How to combine/add two Beta Distribution curves?

Hello, I am currently working on Beta Distribution, and I am using the distribution to model a knowledge/opinion into the software. The user adjusts the alpha and beta values of Beta...

12 September 2020 6,569 5 View