Beta carotene by hplc?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,688 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,620 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,289 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,781 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,331 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,083 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

Beta cyclodextrin complex characterization methods?

Which is the best method for the characterization of beta cyclodextrin complex formation with guest molecule in solution form and in solid form ?

11 June 2024 6,719 0 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

How Can I Understand the Different Results from MacConkey Agar and β-Galactosidase Assays in a LexA-Based Bacterial-Two Hybrid System?

I'm conducting protein-protein interaction studies using a LexA-based Bacterial-Two Hybrid system with E. coli reporter strains. We employ MacConkey agar and β-galactosidase assays to evaluate the...

26 May 2024 4,647 0 View

Validation of the formula for beta-carotene estimation?

For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm...

16 April 2024 3,739 1 View

How can I solve a problem with the amplification of V4 primers for algae?

Hello everyone I need your help with a problem I can't seem to solven : I'm planning to do some sequencing of freshwater algae. So I referred to the primer pair made by Stoeck et al. 2010 and...

23 January 2024 5,970 4 View

How to trypsinize IMR90 cells to get good viability?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

18 January 2024 958 1 View

I have resuspended lyophilised Amyloid beta protein in Guandine HCL, how should I store it for long term use?

We are purifying the protein from recombinant DNA

05 January 2024 1,086 2 View

Can I get the standard FTIR spectra of beta-carotene, betanin, anthocyanin?

I am struggling with the standard spectra of beta-carotene, betanin, anthocyanin components.. please anyone help me out. I shall be very much thankful!

25 December 2023 5,232 1 View