Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

Key elements to consider before rolling out the final survey?

hi, for my quantitative research, i am analysing the pilot survey data. While, my cronbach is looking good, but Tstat, few outer loadings & Pval doesnt look good. Which are the key...

01 May 2020 6,426 10 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Unit of power in line spectrum from conversion from log scale(dB) to linear scale(?) ?

I have a doubt about the unit of amplitude on y axis on linear plot of a sound wave generated in Matlab. In logarithmic scale it is dB but I am confused about what it should be on the linear scale...

04 September 2019 6,644 4 View

Which turbulence model give best results to capture flow characteristics in S shaped diffuser duct in context of ansys fluent?

computational fluid dynamics problem

03 September 2018 1,128 4 View

Which solvent system(s) can specifically be used to elute triterpenoids from leaf extracts using column chromatography?

I am using Chl:meth system to elute the triterpenoids through column chromatography using silica 60-120 mesh. But high amount of impurities are coming with the desired molecule. If the molecule...

25 April 2018 7,287 3 View

I have extracted plant phenolics in aqueous methanol and want to prepare solution for gc-ms analysis to quantify phenolics. Help me in prep solution?

I want to quantify phenolics present in my aq. methanol extract using gc ms. But i want to make the extaract volatile so that it could separate out the phenolics. Please guide me to prepare my...

17 April 2018 6,145 4 View

Can bacteriophages be used against biofilm forming within indwelling medical devices in-vivo?

14 August 2017 4,098 3 View

What are superconducting fluctuation?

https://journals.aps.org/pr/abstract/10.1103/PhysRev.156.396 States that "Fluctuation occurs which drives the amplitude of the order.parameter to zero at all points on a surface which seversthe...

18 June 2017 7,684 17 View

How much area is required to set up 10 MW parabolic trough collector solar thermal plant?

i want to compare land area requirement for power tower and parabolic trough case of mentioned capacity. If any literature available please share. assuming 800kw/m2 solar irradiation; 300 sunny...

21 August 2016 1,405 13 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View