Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group and the senescence induced group had a worse stain. I have raised many questions about it staining endogenous lysosomes, also changing pH, osmolarity (fixative time)... I have tried all kinds of methods and followed the protocol as well but my results are not feasible..

Thanks a lot.