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I'm working on enzyme beta glucosidase. I used CMC in my production media and the activity starts coming after 5 hours but after 24 hrs no activity is coming. Has anybody experienced the same problem?
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I've clone a gene of Beta glucosidase enzyme in E.coli. Most of my protein is going in inclusion bodies. So I tried the urea treatment for the solubilization of inclusion bodies and afterwards...
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