Hi
We are planning to perform ELISA for CD39 and CD73 using kidney tissue homogenates. Does anyone have experience in this area in preparing the tissue samples?
Thanks.
Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...
09 October 2019 9,198 4 View
Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...
06 July 2019 1,293 10 View
Hi I am trying to develop positive control for protein, lipid and DNA as a measure of oxidative stress in formalin fixed paraffin embedded normal tissues during immunohistochemistry. For protein...
09 October 2017 6,056 2 View
Hi, My query is regarding the amount of DNA in my sample using Picogreen assay kit. I normally use a total reaction volume of 100 microlitre out of which 50 microlitre is 1:200 fold diluted...
07 August 2016 8,576 7 View
Hi, I have started using the JACoP plugin of Image J and found it to have an user friendly interface. Before using the Plugin, I select two channels for my image of interest (I prefer to use...
05 June 2016 6,871 0 View
Hi, is there any source of mouse, rat, pig and human intestinal cell line which behaves like primary cultures or exhibit character similar to them when cultured? IPEC-J2 cell line from pig seems...
03 April 2016 3,172 5 View
Hi, I am trying to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform. I always find the bluish color of the organic layer to be...
02 March 2016 2,715 5 View
Hi I am wondering if anybody have had experiences with Opal 4 or 7 colour multiplex IHC. Since, it is based on Tyramide amplification, I am interested to know the experience with this technique...
01 January 1970 2,232 0 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View