Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

09 October 2019 9,111 4 View

RNA stability at different temperature?

Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...

06 July 2019 1,220 10 View

How to induce lipid damage?

Hi I am trying to develop positive control for protein, lipid and DNA as a measure of oxidative stress in formalin fixed paraffin embedded normal tissues during immunohistochemistry. For protein...

09 October 2017 5,989 2 View

ELISA FOR CD39 AND 73

07 August 2017 242 0 View

Picogreen assay quantification of samples ?

07 August 2016 8,490 7 View

Background substraction before colocalization analysis using JACoP?

Hi, I have started using the JACoP plugin of Image J and found it to have an user friendly interface. Before using the Plugin, I select two channels for my image of interest (I prefer to use...

05 June 2016 6,783 0 View

Intestinal cell line behaving like primary cultures.

03 April 2016 3,106 5 View

How to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform?

Hi, I am trying to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform. I always find the bluish color of the organic layer to be...

02 March 2016 2,624 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View