Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

09 October 2019 9,111 4 View

RNA stability at different temperature?

Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...

06 July 2019 1,220 10 View

How to induce lipid damage?

Hi I am trying to develop positive control for protein, lipid and DNA as a measure of oxidative stress in formalin fixed paraffin embedded normal tissues during immunohistochemistry. For protein...

09 October 2017 5,989 2 View

ELISA FOR CD39 AND 73

07 August 2017 242 0 View

Picogreen assay quantification of samples ?

07 August 2016 8,490 7 View

Background substraction before colocalization analysis using JACoP?

Hi, I have started using the JACoP plugin of Image J and found it to have an user friendly interface. Before using the Plugin, I select two channels for my image of interest (I prefer to use...

05 June 2016 6,783 0 View

How to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform?

Hi, I am trying to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform. I always find the bluish color of the organic layer to be...

02 March 2016 2,624 5 View

Opal 4 or 7 colour IHC and the pitfalls?

01 January 1970 2,165 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View