9 Questions 13 Answers 0 Followers
Questions related from Debashish Banerjee
Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...
10 October 2019 9,173 4 View
Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...
07 July 2019 1,267 10 View
Hi I am trying to develop positive control for protein, lipid and DNA as a measure of oxidative stress in formalin fixed paraffin embedded normal tissues during immunohistochemistry. For protein...
10 October 2017 6,028 2 View
Hi We are planning to perform ELISA for CD39 and CD73 using kidney tissue homogenates. Does anyone have experience in this area in preparing the tissue samples? Thanks.
08 August 2017 281 0 View
Hi, My query is regarding the amount of DNA in my sample using Picogreen assay kit. I normally use a total reaction volume of 100 microlitre out of which 50 microlitre is 1:200 fold diluted...
08 August 2016 8,546 7 View
Hi, I have started using the JACoP plugin of Image J and found it to have an user friendly interface. Before using the Plugin, I select two channels for my image of interest (I prefer to use...
06 June 2016 6,842 0 View
Hi, is there any source of mouse, rat, pig and human intestinal cell line which behaves like primary cultures or exhibit character similar to them when cultured? IPEC-J2 cell line from pig seems...
04 April 2016 3,148 5 View
Hi, I am trying to measure sodium dodecyl sulphate after incubation with methylene blue and subsequent extraction with chloroform. I always find the bluish color of the organic layer to be...
03 March 2016 2,684 5 View
Hi I am wondering if anybody have had experiences with Opal 4 or 7 colour multiplex IHC. Since, it is based on Tyramide amplification, I am interested to know the experience with this technique...
01 January 1970 2,210 0 View
