Is this true for pseudomonas aeruginosa? or other bacteria?
Short answer, yes...metabolic byproducts secreated into the medium can not only lower pH but increase it as well...it just depends on factors such as what bug is growing, what it is growing in, length of time in culture, etc.
Hi Researchgate folks, Our lab has mouse FFPE tissue which has cells that express a TdTomato reporter as well has a FAM labeled drug as we routinely use to test the delivery of the drug to the...
15 April 2024 6,632 0 View
Hello, I am new to working with human microvascular endothelial cells (bought from Sigma), and there is varying information on how many passages you can take these cells to and still get a...
26 September 2023 375 2 View
I want to coat APTES on pHEMA thin film. I have prepared a Silanization solution (5% APTES in 95% EtOH) and the pH was adjusted to 5 by acetic acid. But it is not work. Can anyone help me provide...
24 May 2023 2,419 2 View
Working conditions were simulated by forward biasing the 1 mm2 solar cells at the same current level (250 mA, i.e. 25 A/cm2) they would handle at the operating concentration (i.e. 1000 suns)’
28 February 2023 736 1 View
Which way does the receptors on Golgi apparatus face? (facing the cytoplasm or the golgi sacs?) Answering this part above is satisfying enough and I’m all gratitude. I’m asking this because I’m...
17 February 2023 4,277 0 View
If, let’s say glutamate and dopamine(both excitatory), both causes depolarisation in post synaptic neurons, how do they convey different information?
27 December 2022 8,906 2 View
Hi Researchers and Scholars We are a chemical research company using CHN 2400 from PerkinElmer for elemental analysis. Due to the Helium global shortage we switch the carrier gas from Helium to...
01 September 2022 1,566 0 View
Got this error while performing docking in Pyrx after running vina. ligands and proteins both are optimized can someone help??
25 July 2021 6,473 4 View
I've read that the traditional cre-lox system is limited by the distance between the loxP sites, and is usually only functional up to about 2 kb. I am looking to use a site-specific recombinase...
24 October 2020 1,230 4 View
I am currently exploring the Petrel software and require some help with downloading any tutorial data or any seismic data for use within the application. It would be wonderful if someone could...
24 March 2020 5,994 10 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View