Hello,
I am new to working with human microvascular endothelial cells (bought from Sigma), and there is varying information on how many passages you can take these cells to and still get a reliable inflammatory response when stimulated. Does anyone have any experience on how these cells may change or how their inflammatory response may change with increased passaging?
And while I'm here, do these cells need a special treatment in the wells when plating (i.e., 12-well, 48-well plates)
Thanks for any help!