Hello Dear Scientists? Phytochemicals VS Synthetic Chemials? Which one better and Why?

Dear I am working on obesity and writing a review article. I want to add the topic that Why Natural products (Phytochemicals) are more beneficial than synthetic ones? I know that they have less...

02 March 2019 693 2 View

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I have done the extraction with 90% Aqueous Ethanol of 25 gms of the sample and then filtered and then evaporated the solvent. After this, I did Lypholizations and after lyophilization, I got 0.5,...

06 July 2017 6,745 0 View

Sticky sample after Lypholization

Dear scientists, I lyophilized my plant samples and after lyophilization, for some sample, I got a very good fine powder but for some samples I got a sticky solid form just like gum(Not fine...

06 July 2017 5,837 0 View

Total Triterpenoid determination.?

04 May 2017 3,628 3 View

Request for articles

hello all, Can any one suggest or send specific articles for quantitative analysis of Triterpenoids (Taraxasterol), by using C18 Column in HPLC-ELSD method ?

02 March 2017 6,830 0 View

Request for Article for (Triterpenoids) taraxasterol and its actate HPLC analysis??

hello every one. Can anyone send an article in which method of gradient elution for triterpenoids like taraxasterol and its acetate has been analysed by HPLC by using different gradient...

02 March 2017 9,122 3 View

Important Question

Dear Scientists, I am confused now. I did extraction from plant with 90 percent ethanol at 40 degree centigrade in reflux condensor, then i evaporated the ethanol in evaporator and also in vaccum...

01 February 2017 1,921 3 View

Important questions request about HPLC

For HPLC analysis of Phenols and Triterpenoids, what concentration of standards and samples solutions (In mg/ml or microgram /ml) for injection into HPLC should be used? I just want to know about...

01 February 2017 5,382 4 View

Hello to all scientists. ?

Can any one provide me some good articles for the determination of Triterpenoids by HPLC-ELSD methodology?

31 December 2016 284 0 View

Hello dear Scientists. How every one doing?

06 July 2016 4,420 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View