hello every one.
Can anyone send an article in which method of gradient elution for triterpenoids like taraxasterol and its acetate has been analysed by HPLC by using different gradient elutions.
I have some papers but their methods is not working.
Your co operation would be highly appreciated.?
?
Because of the nature of your sample matrix (interferences) you may have to develop and validate your own gradient method. You can adapt these gradients for your sample.
Hello Mr, i have a question about HPLC/UV detection.
how can I know that analyte went through the column without giving retention time althought i got a peak but i do not kniow if this peak belongs to the analyte or not.
You don't (unless you have an MS detector)! You can adjust the composition of the mobile phase and see if the retention time of the sample moves in relation to the reference standard.
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