Hello Dear Scientists? Phytochemicals VS Synthetic Chemials? Which one better and Why?

Dear I am working on obesity and writing a review article. I want to add the topic that Why Natural products (Phytochemicals) are more beneficial than synthetic ones? I know that they have less...

02 March 2019 693 2 View

Does filteration effects the crude extracts activity?

Dear Scientists, I applied the pure crude extracts (without filtration by syringe filter) on colon cancer cell lines and got some good results. When I repeated the experiment with filtration by...

10 November 2017 6,844 0 View

No title

I have done the extraction with 90% Aqueous Ethanol of 25 gms of the sample and then filtered and then evaporated the solvent. After this, I did Lypholizations and after lyophilization, I got 0.5,...

06 July 2017 6,745 0 View

Sticky sample after Lypholization

Dear scientists, I lyophilized my plant samples and after lyophilization, for some sample, I got a very good fine powder but for some samples I got a sticky solid form just like gum(Not fine...

06 July 2017 5,837 0 View

Total Triterpenoid determination.?

04 May 2017 3,628 3 View

Request for articles

hello all, Can any one suggest or send specific articles for quantitative analysis of Triterpenoids (Taraxasterol), by using C18 Column in HPLC-ELSD method ?

02 March 2017 6,830 0 View

Request for Article for (Triterpenoids) taraxasterol and its actate HPLC analysis??

hello every one. Can anyone send an article in which method of gradient elution for triterpenoids like taraxasterol and its acetate has been analysed by HPLC by using different gradient...

02 March 2017 9,122 3 View

Important Question

Dear Scientists, I am confused now. I did extraction from plant with 90 percent ethanol at 40 degree centigrade in reflux condensor, then i evaporated the ethanol in evaporator and also in vaccum...

01 February 2017 1,921 3 View

Important questions request about HPLC

For HPLC analysis of Phenols and Triterpenoids, what concentration of standards and samples solutions (In mg/ml or microgram /ml) for injection into HPLC should be used? I just want to know about...

01 February 2017 5,382 4 View

Hello to all scientists. ?

Can any one provide me some good articles for the determination of Triterpenoids by HPLC-ELSD methodology?

31 December 2016 284 0 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View