I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I have genotyped my RAG mice and they don't express any CD4, CD8 or B cells. I will check mycoplasma contamination but would like to know if there are any other things I should look for !! Thanks in advance
Hi Mona,
I think it’s all about functions of melanoma cell-intrinsic PD-1 receptor, which promotes tumor growth. Namely, the ligation of melanoma-PD-1 to its predominant ligand, PD-L1, as the latter is required for PD-1-driven tumorigenesis. Thus to examine if PD-L1 expressed by melanoma cells contributes to melanoma-PD-1-dependent tumorigenesis, they treated PD-L1(−/−) KO Rag(−/−) vs. wildtype Rag(−/−) mice grafted with Pdcd1-OE melanomas with a PD-L1 blocking antibody. Authors found that PD-L1 blockade inhibited Pdcd1-OE B16-F10 melanoma growth compared to isotype control antibody treatment in PD-L1(−/−) KO mice. Additionally, PD-L1 antibody treatment resulted in significantly reduced tumor growth of Pdcd1-OE melanomas in PD-L1(−/−) KO Rag(−/−) compared to wildtype Rag(−/−) mice. All the above suggest growth-accelerating functions not only of host-PD-L1:melanoma-PD-1 but also of melanoma-PD-L1:melanoma-PD-1 interactions. So, Mona, please test how functional the PD-1 receptor is in your B16F10 melanoma cell line.
Best wishes,
Ilya
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