I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !
Have you tried HFIP?
If its from a commercial source, try contacting their support team to get more info as to how they got the lyophilized powder
Dear Mona,
Have you used methanol:Chloroform in ration of 3:1, May be it will work
best
thanks guys ... I will try HFIP and methanol:chloroform .... I hope they work out.
You can try sarkosyl with & without alkali (pH>~10.
I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...
06 July 2019 5,353 2 View
I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...
05 June 2019 7,168 4 View
How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...
11 December 2017 1,143 0 View
I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...
08 September 2017 9,439 3 View
I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...
05 June 2017 2,072 3 View
I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...
04 May 2017 9,115 0 View
a protocol for in vitro T cells cytotoxicity (killing) assay using CFSE dye.
10 November 2016 3,533 2 View
In-vivo cytotoxicity assay using pulsed and uplused cells with different concentrations of CFSE dye.
08 September 2016 6,635 0 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View