Mona Mohsen

9 Questions 12 Answers 0 Followers

Questions related from Mona Mohsen

Does 4T1 murine breast cancer cell line express any identified tumor antigen to function as a therapeutic target for vaccine development ?

I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...

07 July 2019 5,416 2 View

What is the best dose, administration route and time line to deplete neutrophils using anti-Ly6G clone 1A8 in tumour setting?

I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...

06 June 2019 7,218 4 View

Does anyone have a protocol to measure PDL1 expression on B16F10 melanoma cell line ?

How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...

12 December 2017 1,195 0 View

What is the most reliable T cell-based assays to confirm immunogenicity of peptides obtained from immunopeptidomics in melanoma ?

I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...

09 September 2017 9,492 3 View

Does anyone know why cancer cell line won't grow nicely in RAG-/- mice !! the tumor doesn't grow as expected and it becomes ulcerated at one point !!

I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...

06 June 2017 2,131 3 View

Does anyone work with the low responder mice strains B10.S or B10.BR!

I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...

05 May 2017 9,169 0 View

I need an efficient protocol for CFSE in vitro T cells cytotoxicity (killing) assay ..... any suggestions please??

a protocol for in vitro T cells cytotoxicity (killing) assay using CFSE dye.

11 November 2016 3,606 2 View

Has anyone done in-vivo cytotoxicity assay using CFSE labelling? i need an advise please?

In-vivo cytotoxicity assay using pulsed and uplused cells with different concentrations of CFSE dye.

09 September 2016 6,691 0 View

I have a lyophilized peptide (p33) conjugated to CpGs - i cant find a suitable solvent to dissolve it ... any suggestions?

I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !

02 February 2016 5,872 4 View