Does 4T1 murine breast cancer cell line express any identified tumor antigen to function as a therapeutic target for vaccine development ?

I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...

06 July 2019 5,353 2 View

What is the best dose, administration route and time line to deplete neutrophils using anti-Ly6G clone 1A8 in tumour setting?

I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...

05 June 2019 7,168 4 View

Does anyone have a protocol to measure PDL1 expression on B16F10 melanoma cell line ?

How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...

11 December 2017 1,143 0 View

What is the most reliable T cell-based assays to confirm immunogenicity of peptides obtained from immunopeptidomics in melanoma ?

I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...

08 September 2017 9,439 3 View

Does anyone know why cancer cell line won't grow nicely in RAG-/- mice !! the tumor doesn't grow as expected and it becomes ulcerated at one point !!

I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...

05 June 2017 2,072 3 View

Does anyone work with the low responder mice strains B10.S or B10.BR!

I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...

04 May 2017 9,115 0 View

I need an efficient protocol for CFSE in vitro T cells cytotoxicity (killing) assay ..... any suggestions please??

a protocol for in vitro T cells cytotoxicity (killing) assay using CFSE dye.

10 November 2016 3,533 2 View

I have a lyophilized peptide (p33) conjugated to CpGs - i cant find a suitable solvent to dissolve it ... any suggestions?

I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !

01 February 2016 5,822 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View