In-vivo cytotoxicity assay using pulsed and uplused cells with different concentrations of CFSE dye.
I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...
06 July 2019 5,442 2 View
I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...
05 June 2019 7,260 4 View
How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...
11 December 2017 1,215 0 View
I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...
08 September 2017 9,523 3 View
I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...
05 June 2017 2,154 3 View
I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...
04 May 2017 9,196 0 View
a protocol for in vitro T cells cytotoxicity (killing) assay using CFSE dye.
10 November 2016 3,639 2 View
I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !
01 February 2016 5,909 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View