Does 4T1 murine breast cancer cell line express any identified tumor antigen to function as a therapeutic target for vaccine development ?

I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...

06 July 2019 5,353 2 View

What is the best dose, administration route and time line to deplete neutrophils using anti-Ly6G clone 1A8 in tumour setting?

I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...

05 June 2019 7,168 4 View

Does anyone have a protocol to measure PDL1 expression on B16F10 melanoma cell line ?

How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...

11 December 2017 1,143 0 View

What is the most reliable T cell-based assays to confirm immunogenicity of peptides obtained from immunopeptidomics in melanoma ?

I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...

08 September 2017 9,439 3 View

Does anyone know why cancer cell line won't grow nicely in RAG-/- mice !! the tumor doesn't grow as expected and it becomes ulcerated at one point !!

I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...

05 June 2017 2,072 3 View

Does anyone work with the low responder mice strains B10.S or B10.BR!

I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...

04 May 2017 9,115 0 View

Has anyone done in-vivo cytotoxicity assay using CFSE labelling? i need an advise please?

In-vivo cytotoxicity assay using pulsed and uplused cells with different concentrations of CFSE dye.

08 September 2016 6,635 0 View

I have a lyophilized peptide (p33) conjugated to CpGs - i cant find a suitable solvent to dissolve it ... any suggestions?

I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !

01 February 2016 5,822 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

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03 March 2021 5,360 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View