I need an efficient protocol for CFSE in vitro T cells cytotoxicity (killing) assay ..... any suggestions please??

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Does 4T1 murine breast cancer cell line express any identified tumor antigen to function as a therapeutic target for vaccine development ?

I am looking for identified MHC-I epitopes in 4T1 murine breast cancer cell line that can be used in a therapeutic vaccine, couldn't find something useful in literature so far ! Any known papers...

06 July 2019 5,442 2 View

What is the best dose, administration route and time line to deplete neutrophils using anti-Ly6G clone 1A8 in tumour setting?

I would like to deplete neutrophils CD11b+Ly6GHi cells from mice during my tumour experiment to study their role in tumour protection with my VLP vaccine. I tried to use 100ug/mouse one day before...

05 June 2019 7,260 4 View

Does anyone have a protocol to measure PDL1 expression on B16F10 melanoma cell line ?

How can I measure the expression of PDL1 on B16F10 melanoma cell line. Do I have to treat the cells first with IFN-g before performing flow cytometry or is it better to measure the expression...

11 December 2017 1,215 0 View

What is the most reliable T cell-based assays to confirm immunogenicity of peptides obtained from immunopeptidomics in melanoma ?

I have done immunopeptidomics for B16F10 melanoma cell line and would like to prioritize my peptides to find an immunogenic target. what is the best in vitro T cell based assay (using the isolated...

08 September 2017 9,523 3 View

Does anyone know why cancer cell line won't grow nicely in RAG-/- mice !! the tumor doesn't grow as expected and it becomes ulcerated at one point !!

I am trying to grow B16F10 melanoma cell line in RAG-/- mice, the process used to work perfectly but now I have some problems with the tumor, it looks ulcerated and doesn't grow the same way !! I...

05 June 2017 2,154 3 View

Does anyone work with the low responder mice strains B10.S or B10.BR!

I am working on a project to increase the efficacy of HBsAg vaccine in low responders, we are looking for a collaborator who can provide us with low responder strain mice. A collaboration with...

04 May 2017 9,196 0 View

Has anyone done in-vivo cytotoxicity assay using CFSE labelling? i need an advise please?

In-vivo cytotoxicity assay using pulsed and uplused cells with different concentrations of CFSE dye.

08 September 2016 6,722 0 View

I have a lyophilized peptide (p33) conjugated to CpGs - i cant find a suitable solvent to dissolve it ... any suggestions?

I have tried DMSO - ETHANOL - UREA - GUINIDINE - ACETIC ACID ... I have tried to change the pH - sonication - heating but nothing is working ... need help please !

01 February 2016 5,909 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How to do Mann-Whitney U test with Bonferroni corrected p-values?

Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...

31 July 2024 6,464 5 View

Bonferroni correction. I have independent t-test, paired t-test and ancova conducted. Which test would require Bonferroni adjustment?

I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...

28 July 2024 7,827 6 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View