Dose-response L-Glutamine in vivo?

I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?

31 December 2016 6,368 1 View

Is there an antibody available yet for phospho-Sestrin2?

I was wondering if there was an antibody available yet (commercially) for phospho-sestrin2 (Thr 232, Ser249 or Ser279). According to the paper attached Sestrin2 should be able to get...

08 September 2016 6,945 2 View

Any advice on the increase in blood leucine after a meal?

Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?

09 October 2015 433 2 View

What is the best antibody for human-mouse HIF-2a detection?

I've tried the novus #NB100-122, got a lot of (aspecific) bands.

06 July 2014 5,695 9 View

No title

06 July 2014 8,065 1 View

Does a specific HIF-2a inhibitor exist?

Pharmacological, not siRNA or others.

06 July 2014 6,620 2 View

Can anyone help with MCT1 and 4 quantification on human samples by western blot?

We want to measure MCT1 and 4 on human muscle samples by the use of western blot. On which exact kDa appears the band? Is this a little higher than the expected 42 kDa (eg 49). Information is much...

04 May 2014 5,134 0 View

Contamination of TRIzol by plastic - any thoughts?

We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...

03 April 2014 3,425 1 View

Electrophoresis - breaking glass cover plates. Any suggestions?

We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...

07 August 2013 7,000 6 View

No title

05 June 2013 1,345 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View