Does anyone know a good paper investigating GLUT4 translocation by (confocal) immunohistochemistry in human/mouse skeletal muscle?

04 April 2013 0 2K Report

If possible by a physiological injection of insulin (no clamps or supraphysiological injections)

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Dose-response L-Glutamine in vivo?

I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?

31 December 2016 6,462 1 View

Is there an antibody available yet for phospho-Sestrin2?

I was wondering if there was an antibody available yet (commercially) for phospho-sestrin2 (Thr 232, Ser249 or Ser279). According to the paper attached Sestrin2 should be able to get...

08 September 2016 7,032 2 View

Any advice on the increase in blood leucine after a meal?

Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?

09 October 2015 590 2 View

What is the best antibody for human-mouse HIF-2a detection?

I've tried the novus #NB100-122, got a lot of (aspecific) bands.

06 July 2014 5,842 9 View

Can you measure D-β-Hydroxybutyrate in human plasma samples stored in -80°C?

The venous samples have been taken in normal EDTA tubes, centrifuged and then stored in -80°C. They have not been DE-proteined by perchloric acid.

06 July 2014 8,178 1 View

Does a specific HIF-2a inhibitor exist?

Pharmacological, not siRNA or others.

06 July 2014 6,757 2 View

Can anyone help with MCT1 and 4 quantification on human samples by western blot?

We want to measure MCT1 and 4 on human muscle samples by the use of western blot. On which exact kDa appears the band? Is this a little higher than the expected 42 kDa (eg 49). Information is much...

04 May 2014 5,231 0 View

Contamination of TRIzol by plastic - any thoughts?

We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...

03 April 2014 3,532 1 View

Electrophoresis - breaking glass cover plates. Any suggestions?

We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...

07 August 2013 7,109 6 View

How does one generate membrane fractions out of frozen (-80°C) muscle biopsies?

I'm looking for a method to generate membrane fractions (not crude membrane, but cell membrane fractions) out of frozen (-80°C) human muscle biopsies. I know it's usally done by sucrose gradient...

05 June 2013 1,448 1 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View