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Questions related from Gommaar D'Hulst
I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?
01 January 2017 6,434 1 View
I was wondering if there was an antibody available yet (commercially) for phospho-sestrin2 (Thr 232, Ser249 or Ser279). According to the paper attached Sestrin2 should be able to get...
09 September 2016 7,002 2 View
Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?
10 October 2015 533 2 View
I've tried the novus #NB100-122, got a lot of (aspecific) bands.
07 July 2014 5,794 9 View
The venous samples have been taken in normal EDTA tubes, centrifuged and then stored in -80°C. They have not been DE-proteined by perchloric acid.
07 July 2014 8,136 1 View
Pharmacological, not siRNA or others.
07 July 2014 6,716 2 View
We want to measure MCT1 and 4 on human muscle samples by the use of western blot. On which exact kDa appears the band? Is this a little higher than the expected 42 kDa (eg 49). Information is much...
05 May 2014 5,204 0 View
We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...
04 April 2014 3,490 1 View
We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...
08 August 2013 7,075 6 View
I'm looking for a method to generate membrane fractions (not crude membrane, but cell membrane fractions) out of frozen (-80°C) human muscle biopsies. I know it's usally done by sucrose gradient...
06 June 2013 1,408 1 View
If possible by a physiological injection of insulin (no clamps or supraphysiological injections)
04 April 2013 2,014 0 View
I normally let the gel harden overnight, but is this necessary? Can I, for example, prepare the gel in the morning and load it with proteins in the afternoon?
04 April 2013 2,847 2 View
