Dose-response L-Glutamine in vivo?

I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?

31 December 2016 6,368 1 View

Is there an antibody available yet for phospho-Sestrin2?

I was wondering if there was an antibody available yet (commercially) for phospho-sestrin2 (Thr 232, Ser249 or Ser279). According to the paper attached Sestrin2 should be able to get...

08 September 2016 6,945 2 View

Any advice on the increase in blood leucine after a meal?

Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?

09 October 2015 433 2 View

What is the best antibody for human-mouse HIF-2a detection?

I've tried the novus #NB100-122, got a lot of (aspecific) bands.

06 July 2014 5,695 9 View

No title

06 July 2014 8,065 1 View

Does a specific HIF-2a inhibitor exist?

Pharmacological, not siRNA or others.

06 July 2014 6,620 2 View

Contamination of TRIzol by plastic - any thoughts?

We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...

03 April 2014 3,425 1 View

Electrophoresis - breaking glass cover plates. Any suggestions?

We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...

07 August 2013 7,000 6 View

No title

05 June 2013 1,345 1 View

Does the time between the making of a home-made (10%) gel and the actual loading of the gel influence the resolution of the bands?

I normally let the gel harden overnight, but is this necessary? Can I, for example, prepare the gel in the morning and load it with proteins in the afternoon?

03 April 2013 2,787 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View