Dose-response L-Glutamine in vivo?

I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?

31 December 2016 6,368 1 View

Any advice on the increase in blood leucine after a meal?

Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?

09 October 2015 433 2 View

What is the best antibody for human-mouse HIF-2a detection?

I've tried the novus #NB100-122, got a lot of (aspecific) bands.

06 July 2014 5,695 9 View

No title

06 July 2014 8,065 1 View

Does a specific HIF-2a inhibitor exist?

Pharmacological, not siRNA or others.

06 July 2014 6,620 2 View

Can anyone help with MCT1 and 4 quantification on human samples by western blot?

We want to measure MCT1 and 4 on human muscle samples by the use of western blot. On which exact kDa appears the band? Is this a little higher than the expected 42 kDa (eg 49). Information is much...

04 May 2014 5,134 0 View

Contamination of TRIzol by plastic - any thoughts?

We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...

03 April 2014 3,425 1 View

Electrophoresis - breaking glass cover plates. Any suggestions?

We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...

07 August 2013 7,000 6 View

No title

05 June 2013 1,345 1 View

Does the time between the making of a home-made (10%) gel and the actual loading of the gel influence the resolution of the bands?

I normally let the gel harden overnight, but is this necessary? Can I, for example, prepare the gel in the morning and load it with proteins in the afternoon?

03 April 2013 2,787 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View