01 January 2017 1 6K Report

I want to do L-Glutamine injections in mice to check mTORC1 activation in muscle. I cannot find dose-response or time-course studies in literature. Could anyone help me out?

More Gommaar D'Hulst's questions See All
Is there an antibody available yet for phospho-Sestrin2?

I was wondering if there was an antibody available yet (commercially) for phospho-sestrin2 (Thr 232, Ser249 or Ser279). According to the paper attached Sestrin2 should be able to get...

08 September 2016 7,032 2 View

Any advice on the increase in blood leucine after a meal?

Does anyone have good reference papers looking at the increase in leucine concentration after a standard meal (leucine gavage) in humans and mice?

09 October 2015 590 2 View

What is the best antibody for human-mouse HIF-2a detection?

I've tried the novus #NB100-122, got a lot of (aspecific) bands.

06 July 2014 5,842 9 View

Can you measure D-β-Hydroxybutyrate in human plasma samples stored in -80°C?

The venous samples have been taken in normal EDTA tubes, centrifuged and then stored in -80°C. They have not been DE-proteined by perchloric acid.

06 July 2014 8,178 1 View

Does a specific HIF-2a inhibitor exist?

Pharmacological, not siRNA or others.

06 July 2014 6,757 2 View

Can anyone help with MCT1 and 4 quantification on human samples by western blot?

We want to measure MCT1 and 4 on human muscle samples by the use of western blot. On which exact kDa appears the band? Is this a little higher than the expected 42 kDa (eg 49). Information is much...

04 May 2014 5,231 0 View

Contamination of TRIzol by plastic - any thoughts?

We recently put a couple of ML of TRIzol in plastic tubes. RNA extraction afterwards yielded contaminated RNA, indicated by a >2,4 260/280 ratio. Is it possible that the storage of TRIzol in...

03 April 2014 3,532 1 View

Electrophoresis - breaking glass cover plates. Any suggestions?

We are doing Western Blots with home made gels. The problem we are having since a couple of months is that the glass cover plates regularly break when gel-electrophoresis is finished. We use a...

07 August 2013 7,109 6 View

How does one generate membrane fractions out of frozen (-80°C) muscle biopsies?

I'm looking for a method to generate membrane fractions (not crude membrane, but cell membrane fractions) out of frozen (-80°C) human muscle biopsies. I know it's usally done by sucrose gradient...

05 June 2013 1,448 1 View

Does the time between the making of a home-made (10%) gel and the actual loading of the gel influence the resolution of the bands?

I normally let the gel harden overnight, but is this necessary? Can I, for example, prepare the gel in the morning and load it with proteins in the afternoon?

03 April 2013 2,879 2 View

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"A Markov-like Model for Patient Progression"?

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