Protocol for PC-3 spheroid (hanging drop) fixation and staining/IF?

Hi everyone, I am currently using the hanging drop method to generate spheroids of PC-3 prostate cancer cells. I would like to know if anyone has a detailed protocol I can use to fix these...

19 February 2020 2,756 1 View

Is it any way to do partition of edges not in same plane in Abaqus?

I am developing a model in Abaqus to simulate the cornea. I now want to partition the solid so as to set the middle part and outside part with different materials. However, I am having difficulty...

11 December 2019 7,645 1 View

Is it necessary to crosslink the RNA and protein while doing RNA binding protein immunoprecipitation?

06 September 2017 9,576 6 View

Reduce hemoglobin in RNA extracted from bird blood for Illumina sequencing

We want to sequence RNA from passerine bird blood to look at host expression. We will need to remove the ribosomal RNA and also the globin RNAs during the library preparation. There is no...

16 July 2017 1,842 4 View

TURBO DNase innactivation using Trizol + chloroform+ isopropanol on RNA suspended in n.f water?

I have extracted RNA from avian blood using TRIzol from ambion. After a 3M sodium acetate / ethanol precipitation, the RIN number reads >8 on the bioanalyzer. I want to treat the RNA with TURBO...

17 May 2017 3,085 5 View

How do I Compare two sets of likert scale data? Perceived and felt emotional responses to music

I am a student who has just conducted a study where I want to compare participants felt and perceived emotional responses when they listen to music. I had 3 conditions which where participants...

11 April 2015 8,969 16 View

Does anyone know the list of genes that regulates calcium ion channel?

Like to do PCR on genes involved in calcium ion signalling pathways specially in neurotransmitters.

24 September 2013 6,642 2 View

How to freeze water into ice without using Liquid Nitrogen or other cryogenic agents?

Well i am preparing ice for my project and to prepare it, we have been using Liq.N2. The current idea is to use a refrigerating unit to maintain the temperature of ice from freezing. But why can't...

11 October 2011 6,985 3 View

No title

02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View