10 Questions 47 Answers 0 Followers
Questions related from Muhammad Imran
Which of the following is the exact way to write an amount/magnitude with it's unit(s)? 6h, 20℃, 20%, 5m etc. (There is no space between the unit and magnitude (number)). OR 6 h, 20 ℃,...
03 March 2022 7,672 7 View
I am going to purchase a deep freezer (-20 C). I have JP Selecta Spain deep freezer offer from a local supplier here...It is comparatively cheap, but I am just curious about the quality. I am...
04 December 2016 2,284 2 View
I am looking for 1,3-Benzodithiole-2-thione. I am going to use it as a standard in HPLC-based Isothiocyanates analysis. Can anyone please suggest any chemicals supplier/company? I need just 1-5...
23 March 2016 189 7 View
I am interested in studying the antiviral potential of Dengue Virus NS3 helicase inhibitors. For the bioassay, I need NS3 helicase/protease. Can anyone please tell me where I can purchase this...
09 July 2014 4,413 4 View
Can anyone please explain the speciifc surface markers expressed by B, T, and NK cells of mouse origin?
28 October 2013 7,226 13 View
I want to activate Akt in human myeloid leukemia cells and insulin treatment is one of the best choices for its activation (Akt phosphorylation). Has anyone ever tried insulin treatment in human...
22 October 2013 2,029 8 View
Suspension cells are hard to transfect, thus making it difficult to overexpress or knockdown a gene. The alternative strategies are either to do electroporation, nucleofection or lentiviral...
13 September 2013 6,765 41 View
I have been doing serum deprivation based studies. My gene of interest is significantly upregulated when cells are serum starved. I have confirmed gene expression by PCR so many times. However, I...
21 June 2013 4,621 22 View
Does anyone know the best method for detection of Mycoplasma contamination in the cell culture system? Also what could be the best approach (drug etc.) to avoid mycoplasma contamination?
10 May 2013 4,184 12 View
I have my gene of interest in the pCDH cDNA Cloning and Expression Lentivectors.I understand that pCDH expression constructs are normally packaged into pseudotyped viral particles (lentiviral...
02 May 2013 7,621 3 View
