I would like to immortalize blood cells and cells from a skin biopsy. I need advice on which protocol to use.
For B cells immortalization from patients, we use the Epstein-Barr virus...
a "general" protocol can be found here:
http://www.unclineberger.org/tissueculture/protocols/b-lymphocytesprotocol
it's similar to our protocol
For skin, I'm sorry, I've never immortalized this kind of cells...but probably it can be done by serial replating of cells (Immortalization by serial passage)...
Hope someone else can help you
same we did LCL from patients that have been transplanted so is the same B cell importalisation using B95 sup virus. see publication
here is the paper
https://www.researchgate.net/publication/9065782_Ex_vivo_priming_of_nave_T_cells_into_EBV-specific_Th1Tc1_effector_cells_by_mature_autologous_DC_loaded_with_apoptoticnecrotic_LCL
Article Ex Vivo Priming of Naive T Cells Into EBV-Specific Th1/Tc1 E...
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