4 Questions 30 Answers 0 Followers
Questions related from Nicola Lo Buono
I've a strange problem: I transformed E.Coli (DH5alpha strain), checked plasmid by PCR, grew them O.N. as mini culture for miniPrep, and freezed them as glycerol stock. Now, I took my glycerol...
21 January 2014 9,213 8 View
I'm looking for a plasmid (any backbone can be ok) encoding for SEAP (Soluble Embryonic Alkaline Phosphatase). I've tried to buy it from plasmid databases (addgene, etc) but my new department...
24 April 2013 3,194 1 View
Have you got a free and really powerful software for managing specimens in freezers? A php script with a mysql database, a free software with a clear GUI or something similar? We're full of books...
22 November 2012 3,335 4 View
Hi everybody! I'm looking for a good protocol to extract DNA from Trizol samples. I usually extraxt RNA with Trizol, but now I'd like to take also gDNA. I used this protocol: 1. After having...
13 November 2010 7,889 17 View