Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

06 June 2013 0 6K Report

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could aluminium hydroxide damage proteins?

Badges
Science method

More Kait Stone's questions See All

Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

31 December 2017 3,934 4 View

Not column based DNA extraction kit for stool/soil samples?

I'm looking for a DNA extraction kit that is beed based. So, far I found only one from Omega. Do I have any other options? Please advice.

04 May 2017 5,207 4 View

Which molecule(s) of LPS bind a Silver Stain?

I'd like to understand some chemistry behind silver staining.

05 June 2014 325 2 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

08 September 2013 9,354 4 View

What essay would you recommend for vaccine reference monitoring?

Qualitative and or quantitative analysis of endotoxin or proteins

06 July 2013 469 0 View

Is there any chemical (acid/base hydrolysis) or else that can be used to degrade LPS?

I just want to see the difference between LPS bands in bacterial cells and degraded LPS bands on an immunoblot.

06 July 2013 9,060 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

04 May 2013 5,625 1 View

How do you develop HRP-conjugated 2 antibody?

I don't have a biotinylated secondary antibody, but have a HRP conjugated one. What do you develop it with?

01 February 2013 6,691 2 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

31 December 2012 7,987 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View