Kait Stone

10 Questions 7 Answers 0 Followers

Questions related from Kait Stone

Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

01 January 2018 3,915 4 View

Not column based DNA extraction kit for stool/soil samples?

I'm looking for a DNA extraction kit that is beed based. So, far I found only one from Omega. Do I have any other options? Please advice.

05 May 2017 5,189 4 View

Which molecule(s) of LPS bind a Silver Stain?

I'd like to understand some chemistry behind silver staining.

06 June 2014 307 2 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

09 September 2013 9,337 4 View

Is there any chemical (acid/base hydrolysis) or else that can be used to degrade LPS?

I just want to see the difference between LPS bands in bacterial cells and degraded LPS bands on an immunoblot.

07 July 2013 9,032 0 View

What essay would you recommend for vaccine reference monitoring?

Qualitative and or quantitative analysis of endotoxin or proteins

07 July 2013 454 0 View

Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...

06 June 2013 5,544 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

05 May 2013 5,606 1 View

How do you develop HRP-conjugated 2 antibody?

I don't have a biotinylated secondary antibody, but have a HRP conjugated one. What do you develop it with?

02 February 2013 6,673 2 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

01 January 2013 7,967 2 View