I just want to see the difference between LPS bands in bacterial cells and degraded LPS bands on an immunoblot.
Salmonella surveillance
31 December 2017 3,934 4 View
I'm looking for a DNA extraction kit that is beed based. So, far I found only one from Omega. Do I have any other options? Please advice.
04 May 2017 5,207 4 View
I'd like to understand some chemistry behind silver staining.
05 June 2014 325 2 View
Qualitative analysis of LPS
08 September 2013 9,354 4 View
Qualitative and or quantitative analysis of endotoxin or proteins
06 July 2013 469 0 View
There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...
05 June 2013 5,563 0 View
Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...
04 May 2013 5,625 1 View
I don't have a biotinylated secondary antibody, but have a HRP conjugated one. What do you develop it with?
01 February 2013 6,691 2 View
I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...
31 December 2012 7,987 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
The recharge of deep sandy groundwater is slow. Does the high level of nitrate promote bacteriological activity knowing that it requires a high level of O2? Thanks
10 July 2024 9,883 5 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View