Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

31 December 2017 3,876 4 View

Not column based DNA extraction kit for stool/soil samples?

04 May 2017 5,147 4 View

Which molecule(s) of LPS bind a Silver Stain?

I'd like to understand some chemistry behind silver staining.

05 June 2014 275 2 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

08 September 2013 9,302 4 View

What essay would you recommend for vaccine reference monitoring?

Qualitative and or quantitative analysis of endotoxin or proteins

06 July 2013 421 0 View

Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...

05 June 2013 5,503 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

04 May 2013 5,568 1 View

How do you develop HRP-conjugated 2 antibody?

I don't have a biotinylated secondary antibody, but have a HRP conjugated one. What do you develop it with?

01 February 2013 6,635 2 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

31 December 2012 7,931 2 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View