Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

31 December 2017 3,876 4 View

Not column based DNA extraction kit for stool/soil samples?

04 May 2017 5,147 4 View

Which molecule(s) of LPS bind a Silver Stain?

I'd like to understand some chemistry behind silver staining.

05 June 2014 275 2 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

08 September 2013 9,302 4 View

What essay would you recommend for vaccine reference monitoring?

Qualitative and or quantitative analysis of endotoxin or proteins

06 July 2013 421 0 View

Is there any chemical (acid/base hydrolysis) or else that can be used to degrade LPS?

I just want to see the difference between LPS bands in bacterial cells and degraded LPS bands on an immunoblot.

06 July 2013 8,956 0 View

Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...

05 June 2013 5,503 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

04 May 2013 5,568 1 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

31 December 2012 7,931 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View