Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

31 December 2017 3,876 4 View

Not column based DNA extraction kit for stool/soil samples?

04 May 2017 5,147 4 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

08 September 2013 9,302 4 View

What essay would you recommend for vaccine reference monitoring?

Qualitative and or quantitative analysis of endotoxin or proteins

06 July 2013 421 0 View

Is there any chemical (acid/base hydrolysis) or else that can be used to degrade LPS?

I just want to see the difference between LPS bands in bacterial cells and degraded LPS bands on an immunoblot.

06 July 2013 8,956 0 View

Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...

05 June 2013 5,503 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

04 May 2013 5,568 1 View

How do you develop HRP-conjugated 2 antibody?

I don't have a biotinylated secondary antibody, but have a HRP conjugated one. What do you develop it with?

01 February 2013 6,635 2 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

31 December 2012 7,931 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View